This course, Basics of Sequencing, offers a foundational understanding of sequencing technologies, with a focus on both Sanger and Next-Generation Sequencing (NGS) methods. Designed to equip learners with essential knowledge in sequencing, the course covers fundamental concepts in DNA and RNA sequencing, the steps involved in library preparation, and the chemistry behind sequencing reactions. Additionally, participants will learn about key quality metrics, including read quality, that are critical for assessing sequencing data accuracy. Ideal for beginners in genomics, this course serves as a stepping stone to more advanced sequencing applications in research and clinical settings.