This course provides an introduction to single-cell RNA sequencing, guiding participants through the unique principles and data analysis techniques involved in studying gene expression at the single-cell level. Participants will learn about single-cell sample preparation, clustering techniques, and methods for dimensionality reduction to visualize high-dimensional data. The course also covers cell type identification and differential gene expression analysis, enabling a deep understanding of cellular heterogeneity and gene regulation within individual cells. This foundational course is ideal for learners looking to explore the powerful applications of single-cell RNA sequencing in genomics and biomedical research.